Fig 1: Antioxidant enzymatic systems in human RBCs.A) Representative Western blot analysis of antioxidant enzymes expressed in the cytosol of human RBCs. Human total erythrocyte lysates, cytosolic fractions and isolated ghost membrane samples (20 µg of proteins each) were analyzed by immunoblotting analysis as described in Materials and Methods; bands specific for SOD1 (at 17 kDa); Catalase (at 60 kDa); GPX1, Prdx1 and 2 (at 22 kDa); and Prdx6 (at 26 kDa) are shown using chemiluminescent HRP-based detection. HUVEC cell lysates were used as positive control (except catalase).B) Representative image of immunofluorescent detection of SOD3 (upper-right panel) and CD235a (or Glycophorin A; lower-left panel) in healthy human RBCs as described in Materials and Methods. The upper-left panel shows the overlay of both immunodetected signals. As negative control (bottom-right panel), RBCs were stained with conjugated secondary antibody only (Alexa Fluor-488 anti-mouse IgG).